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技术文章

Rabbit Anti-CD144/VECD/VE Cadherin抗体说明书

阅读:462发布时间:2013-4-24

Rabbit Anti-CD144/VECD/VE Cadherin抗体说明书

FROM: ABCAM

Cat. Number:  RS-0878R

Quantity size:  100ul  (dilute with pH 7.4    0.01 M PBS or diluent of antibody)

Background: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Specificity:       ·anti-CD144 is a rabbit polyclonal antibody unconjugated

                ·specific for CD144 of  hu, rat and mo

                · use for western blotting, elisa, immunoprecipitation    

                   and immunohistochemistry

·  Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 82kDa

Application:     · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

·  Optimal working dilutions must be determined by the end user.

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.

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