试剂盒名称:髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG(MPO-ANCA IgG)
军团菌抗体IgA
规格: 96T/48T
品牌:BIOFINE
种属:人ELISA试剂盒
检测波长:450nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃，六个月，-20℃一年
检测目的:用于测定血清，血浆及相关液体髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG(MPO-ANCA IgG)含量。适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
ELISA实验小知识：ELISA检测试剂盒标本的稀释原则：首先通过文献检索的方式了解待测样本的大致含量，确定适当的稀释倍数。只有稀释至标准曲线的范围内，检测的结果才是准确的。稀释的过程中，应做好详细的记录。zui后计算浓度时，稀释了“N”倍，标本的浓度应再乘以“N”。
髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG(MPO-ANCA IgG)Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG(MPO-ANCA IgG)
组织蛋白酶D(cath-D)
破伤风抗体(Tetanus Ab)
中性粒细胞弹性蛋白酶(NE)
免疫球蛋白G Fc段受体Ⅲ(FcγRⅢ/CD16)
血红素氧合酶2(HO-2)
抗Sc1-70抗体(Sc1-70-Ab)
EB病毒IgA(EBv IgA)
干扰素调节因子(IRF)
胃肠癌标志物CA199
钙联蛋白(CNX)
晚期糖基化终末产物受体(RAGE/AGER)
肺癌标志物DR-70(DR-70TM)
