PCDH1
试剂盒名称:1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)ELISA试剂盒
英文名：Human protocadherin 1,PCDH1
品牌:BIOFINE
种属:大鼠ELISA试剂盒
检测波长:450nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃，六个月，-20℃一年
检测目的:用于测定血清，血浆及相关液体1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)含量。适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test. History Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies. In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present in the sample. Radioimmunoassay was first described in a paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960.[5] Because radioactivity poses a potential health threat, a safer alternative was sought. A suitable alternative to radioimmunoassay would substitute a non-radioactive signal in place of the radioactive signal. When enzymes (such as peroxidase) react with appropriate substrates (such as ABTS or 3,3’,5,5’-Tetramethylbenzidine), a change in color occurs, which is used as a signal. However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody. This linking process was independently developed by Stratis Avrameas and G.B. Pierce.[6] Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container; i.e., the immunosorbent has to be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966.[7] In 1971, Peter Perlmann and Eva Engvall at Stockholm University in Sweden, and Anton Schuurs and Bauke van Weemen in The Netherlands independently published papers that synthesized this knowledge into methods to perform EIA/ELISA.[8][9]
1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)由于各种抗原成份，包括小分子的半抗原，均可用以制备特异性的抗血清或单克隆抗体，利用此抗体作为试剂就可检测标本中相应的抗原，因此免疫测定的应用范围极广，在临床检验中可用于测定：1) 体液中的各种蛋白质，包括含量极少的蛋白质如甲胎蛋白等。2) 激素，包括小分子量的甾体激素等。3) 抗生素和药物。4) 病原体抗原，HBsAg、HBeAg等。5) 另外，也可利用纯化的抗原检测标本中的抗体，例如抗-HBs等。
1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)北京
IPO-38蛋白(IPO38)
骨成型蛋白4(BMP-4)
小鼠γ干扰素(IFN-γ)
*8(KLK 8)
脂联素(ADP)
膀胱癌抗原(UBC)
5核苷酸酶(5-NT)
多效生长因子(PTN)
胎盘生长因子(PLGF)
丁型肝炎IgG(HDV IgG)
基质金属蛋白酶抑制因子1(TIMP-1)
血管紧张素Ⅱ受体Ⅰa型(AgtrⅠa)
