ELISA是以免疫学反应为基础，将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
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猪表面活性物质关联蛋白D(SPD)检测盒
Pig Surfactant Associated Protein D (SPD) ELISA
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猪表面活性物质关联蛋白D(SPD)检测盒Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
猪表面活性物质关联蛋白D(SPD)检测盒
猪钙调素(RGN)检测盒Pig Regucalcin (RGN) ELISA
人含跨膜Emp24蛋白运输域蛋白1(TMED1)测定盒Human Transmembrane Emp24 Protein Transport Domain Containing Protein 1 (TMED1) ELISA
猪载脂蛋白A5(APOA5)检测盒Pig Apolipoprotein A5 (APOA5) ELISA
人IK细胞因子(IK)测定盒Human IK Cytokine, Down Regulator Of HLA II (IK) ELISA
猪血小板反应蛋白解整合素金属肽酶4(ADAMTS4)检测盒Pig A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) ELISA
猪摄食抑制因子1(NES1)检测盒Pig Nesfatin 1 (NES1) ELISA
人早期B-细胞因子3(EBF3)测定盒Human Early B-Cell Factor 3 (EBF3) ELISA
猪耦合因子6(CF6)检测盒Pig Coupling Factor 6 (CF6) ELISA
猪红细胞衍生核因子2样蛋白2(NFE2L2)检测盒Pig Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA
猪肿瘤坏死因子α诱导蛋白2(TNFαIP2)检测盒Pig Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) ELISA
猪骨成型蛋白2(BMP2)检测盒Pig Bone Morphogenetic Protein 2 (BMP2) ELISA
猪载脂蛋白B48(APOB48)检测盒Pig Apolipoprotein B48 (APOB48) ELISA
