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大鼠胰岛素样生长因子2(IGF-2)检测试剂盒 IGF-2(R)ELISA kit 酶联免疫双抗夹心法 S/F/T/CC
大鼠胰岛素样生长因子2(IGF-2)检测试剂盒 IGF-2 (R)ELISA kit
规格 96T/48T
特异性 大鼠IGF-2
检测标本 大鼠血清、体液、组织匀浆或细胞培养上清
检测范围 62.4→4000pg/ml
保存条件 4℃六个月，-20℃一年。
内容 1.重组大鼠IGF-2 冻干标准品，10ng/管，总2管。
2.预包被抗大鼠IGF-2 抗体的96孔板。
3.样品稀释液，30ml。
4.*标记抗大鼠IGF-2 ，130μl，效价1：100。
5.抗体稀释液，12ml。
6.亲和素-过氧化物酶复合物(ABC)，130μl，效价1：100。
7.ABC稀释液，12ml。
8.TMB显色液，10ml。
9.TMB终止液，10ml。
背景资料 Insulin-like growth factor 2, IGF-II。胰岛素样生长因子-2。和胰岛素、IGF-1有很高的同源性。IGF-I，IGF-II在很多组织和细胞都有分布。和IGF-I不同的是，IGF-II促进分裂生长的作用*于胚胎时期。成熟形态有67个氨基酸，分子量7.4KDa，和IGF-I有高度的同源性
ELISA试剂盒常见组成部分：
1）大鼠胰岛素样生长因子2(IGF-2)检测试剂盒酶和底物：在ELISA中zui常用的酶为HRP和ALP。
2）抗体：在ELISA中应用的抗体可分为多克隆抗体(多抗)和单克隆抗体(单抗)。
3）抗原：在ELISA中应用的抗原要求有较高的特异性、亲和力和纯度。主要有三类，即自然抗原、人工合成抗原和基因重组抗原。
4 大鼠胰岛素样生长因子2(IGF-2)检测试剂盒包被：将免疫活性物质(抗原或抗体)结合于固相载体上的过程称为包被。常用的材料有聚苯乙烯、硝酸纤维薄膜等；常用的方法有吸附法、化学交联法及亲和素-*间接包被法。
5）固相载体：固相载体是ELISA中用以分离结合标记物和游离标记物的主要手段，应与各种免疫活性物质有良好的结合性能并且不改变其免疫活性。常用的固相载体有聚苯乙烯塑料和硝酸纤维素膜。
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