更多ELISA试剂盒产品
人胰岛素样生长因子1受体(IGF-1R)检测试剂盒 人胰岛素样生长因子Ⅰ受体(IGF-ⅠR)检测试剂盒 人胰岛素样生长因子1受体(IGF-R1)检测试剂盒 IGF-1R(h)ELISA kit 酶联免疫双抗夹心法 S/P/C Human IGF-1R ELISA Kit
IGF1R; IGF 1R; hyft; CD221
人胰岛素样生长因子1受体(IGF-R1)检测试剂盒IGF-1R (h)ELISA kit
规格 96T/48T
特异性 人IGF-1R
检测标本 人血清、血浆、细胞
检测范围 156→10000pg/ml
保存条件 4℃六个月，-20℃一年。
内容 1.重组人IGF-1R冻干标准品，10ng/管，总2管。
2.预包被抗人IGF-1R抗体的96孔板。
3.样品稀释液，30ml。
4.*标记抗人IGF-1R，130μl，效价1：100。
5.抗体稀释液，12ml。
6.亲和素-过氧化物酶复合物(ABC)，130μl，效价1：100。
7.ABC稀释液，12ml。
8.TMB显色液，10ml。
9.TMB终止液，10ml。
背景资料 IGF-1R,胰岛素样生长因子受体1，是一种糖蛋白质。可结合IGF-1和 IGF-2。IGF-1R合成时是一条单链，酶切成一条α 链和一条跨膜β 链，二条α 链和二条β 链构成受体。在很多组织和细胞都有分布。本试剂盒所用的标准品是基因重组表达产物，一条细胞外部分单链，一条α 链和一条细胞外β 链。
ELISA试剂盒常见组成部分：
1）人胰岛素样生长因子1受体(IGF-R1)检测试剂盒酶和底物：在ELISA中zui常用的酶为HRP和ALP。
2）抗体：在ELISA中应用的抗体可分为多克隆抗体(多抗)和单克隆抗体(单抗)。
3）抗原：在ELISA中应用的抗原要求有较高的特异性、亲和力和纯度。主要有三类，即自然抗原、人工合成抗原和基因重组抗原。
4 人胰岛素样生长因子1受体(IGF-R1)检测试剂盒包被：将免疫活性物质(抗原或抗体)结合于固相载体上的过程称为包被。常用的材料有聚苯乙烯、硝酸纤维薄膜等；常用的方法有吸附法、化学交联法及亲和素-*间接包被法。
5）固相载体：固相载体是ELISA中用以分离结合标记物和游离标记物的主要手段，应与各种免疫活性物质有良好的结合性能并且不改变其免疫活性。常用的固相载体有聚苯乙烯塑料和硝酸纤维素膜。
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