中英文说明丨InVivoMAb 抗小鼠 CSF1R (CD115)

The AFS98 monoclonal antibody reacts with mouse colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115. CSF1R is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. In mice CSF1R is expressed by monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. CSF1R is a receptor for CSF1 and CSF1 signaling through CSF1R regulates the proliferation and differentiation of cells in the monocytic lineage. The AFS98 antibody has been reported to deplete macrophages and block CSFR1 in vivo.

Isotype Rat IgG2a, κ

Recommended Isotype Control(s) InVivoMAb rat IgG2a isotype control, anti-trinitrophenol

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Not available or unknown

Reported Applications in vivo macrophage depletion

in vitro CSF1R neutralization

in vivo monocyte depletion

Flow cytometry

Western blot

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 µm filtration

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_2687699

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

AFS98单克隆抗体与小鼠集落刺激因子1受体（CSF1R）（也称为巨噬细胞集落刺激因子受体（M-CSFR））和CD115发生反应。CSF1R是一种单程I型膜蛋白，是血小板衍生生长因子受体家族的成员。在小鼠中，CSF1R由单核细胞/巨噬细胞，腹膜渗出物细胞，浆细胞样和常规树突状细胞以及破骨细胞表达。CSF1R是CSF1的受体，通过CSF1R的CSF1信号传导调节单核细胞谱系中细胞的增殖和分化。据报道，AFS98抗体可消耗巨噬细胞并阻断体内CSFR1。

艾美捷InVivoMAb 抗小鼠 CSF1R (CD115)#BE0213基本参数：

同种型 大鼠IgG2a， κ

推荐的同种型对照 体内MAb大鼠IgG2a同种型对照，抗三-硝-基-苯-酚

推荐的稀释缓冲液 体内纯 pH 7.0 稀释缓冲液

免疫原 不可用或未知

报告的应用程序 体内巨噬细胞耗竭

体外脑脊液1R中

和 体内单核细胞去除

流式细胞术

蛋白质印迹

配方 PBS，pH 7.0

不含稳定剂或防腐剂

内毒素 <2EU/mg （<0.002EU/μg）

通过LAL凝胶凝血测定法测定

纯度 >95%

由 SDS-PAGE 决定

无 菌 0.2 μm 过滤

生产 从无动物设施中的组织培养上清液中纯化

纯化 蛋白 G

里德 AB_2687699

分子量 150 千达

存储 抗体溶液应以4°C的储备浓度储存。 不要冻结。

InVivoMAb 抗小鼠 CSF1R (CD115)部分文献引用：

in vivo macrophage depletion

Kaminsky, L. W., et al. (2015). "Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection" J Virol 89(19): 9974-9985.

in vitro CSF-R1 neutralization

Sheng, K. C., et al. (2014). "IL-3 and CSF-1 interact to promote generation of CD11c+ IL-10-producing macrophages" PLoS One 9(4): e95208.

in vivo monocyte depletion

Greter, M., et al. (2012). "GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells" Immunity 36(6): 1031-1046.