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技术文章

Anti-BrdU抗体说明书

阅读:225发布时间:2013-12-20

Anti-BrdU

Catalog Number :RS-0489R

Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Background:

The immunocytochemicaldetection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful toolto study the cytokinetics of normal and neoplastic cells. In vitro or in vivolabeling of tumor cells with the thymidine analogue BrdU and the subsequentdetection of incorporated BrdU with specific anti-BrdU monoclonal antibodies isan accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells mayprovide an estimate for the fraction of cells in S-phase. Also dynamicproliferative information such as the S-phase transit rate and the potentialdoubling time can be obtained, by means of bivariate BrdU/DNA flow cytometricanalysis

Proliferation Marker

pecificity:   

- Anti-BrdU is a rabbit  monoclonal  antibody unconjugated                

specific for  BrdU of human, mouse, ratcow     

        use for westernblotting,elisa,immunoprecipitation and immunohistochemistry

        Protein G affinity chromatographypurification, purity :>95%

        Isotype:  IgG

        mol wt:  12.5 kDa

Application :  

        WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

       not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at roomtemperature for at least one month and for greater  than a year when kept at -20.When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4

 

Important Note: Thisproduct as supplied is intended for research use only, not for use in  human, therapeutic or diagnosticapplications.


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