Rabbit Anti-MICA抗体WB IHC antibody
FROM:ABCAM
Catalog Number :RS-1610R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: background:
The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
Function:
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Specificity : Anti-MICA抗体WB IHC *
–Anti-MICA is a rabbit polyclonal antibody
–specific for MICA of rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 43 kDa
Application :
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- IF=1:150-200
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃Anti-MICA抗体WB IHC *
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
