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技术文章

Anti-APOA2抗体使用说明

阅读:302发布时间:2013-12-20

 Anti-APOA2

Catalog Number : Rs-5034R

Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBSor antibody diluent )

Background:

Apolipoprotein A II is the second most abundant proteinof the high density lipoprotein particles. The apolipoprotein A II geneconsists of 4 exons and 3 introns. The four exons encode the 5' untranslatedregion, pre peptide, a short N terminal domain and a C terminal domain composedof a variable number of lipid binding amphipathic helices. Familial apolipoproteinA II deficiency may result from a splice junction alteration which blockssplicing of intron 3 from the primary transcript and result in the formation ofa non functional mRNA.Subunit : Homodimer; disulfide-linked. Also forms adisulfide-linked heterodimer with APOD. Interacts with HCV core protein.Interacts with APOA1BP and NDRG1.

Subcellular Location : Secreted.Tissue Specificity :Plasma; synthesized in the liver and intestine.Similarity : Belongs to theapolipoprotein A2 family.

pecificity :    

Anti-APOA2 isa Rabbit polyclonal antibodyunconjugated                

specific for APOA2 of Human,Mouse, Rat, Dog,

    use for westernblotting,elisa,immunoprecipitation and immunohistochemistry

    Protein G affinity chromatographypurification, purity :>95%

    Isotype:  IgG

    mol wt:11kDa

Application :  

     WB=1:100-500 ELISA=1:500-1000 IP=1:20-100  IHC-P=1:100-500 IHC-F=1:100-500  IF=1:100-500
not yet tested in other applications.

     optimaldilutions/concentrations should be determined by the end user..

Storage: Store at -20 for one year. Avoid repeated freeze/thawcycles. The  lyophilized antibody isstable at room temperature for at least one month and for greater  than a year when kept at -20. Whenreconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for atleast six weeks at 2-4

Important Note: Thisproduct as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.

gnosticapplications.


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